Liquid chromatography is a widely used analytical technique in the field of chemistry, biochemistry, and pharmaceuticals. It is a separation technique that separates the components of a mixture based on their interactions with a stationary phase and a mobile phase. Liquid chromatography apparatus for laboratory use is a sophisticated instrument that is used to perform this separation process. The liquid chromatography apparatus consists of several components, including a column, a pump, a detector, and an injector. The column is the heart of the apparatus, where the separation of the mixture occurs. It contains a stationary phase, which is usually a solid material, and a mobile phase, which is a liquid or a gas. The pump is used to push the mobile phase through the column, and the detector is used to detect the separated components as they exit the column. The injector is used to introduce the sample into the mobile phase. There are several types of liquid chromatography apparatus available for laboratory use, including high-performance liquid chromatography (HPLC), ion chromatography (IC), and size exclusion chromatography (SEC). HPLC is the most commonly used technique, which uses a high-pressure pump to push the mobile phase through the column at a high flow rate. IC is used to separate ions based on their charge, while SEC is used to separate molecules based on their size. The liquid chromatography apparatus for laboratory use is an essential tool for chemical analysis and research. It provides high-resolution separation of complex mixtures and enables the identification and quantification of individual components. It is widely used in the pharmaceutical industry for drug development and quality control, in environmental analysis for the detection of pollutants, and in biochemistry for the separation of proteins and other biomolecules.
liquid chromatography, separation technique, stationary phase, mobile phase, high-performance liquid chromatography
CITATION : "David Anderson. 'Liquid Chromatography Apparatus For Laboratory Use.' Design+Encyclopedia. https://design-encyclopedia.com/?E=364191 (Accessed on June 07, 2025)"
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